for Neisseria gonorrhoeae Ann E. Jerse, Ph.D. Professor Department of Microbiology and Immunology F. Edward Hébert School of Medicine Uniformed Services University Bethesda, Maryland, USA WHO PD-VAC Meeting June 21st-22nd, 2017

The Gram staining test (Gram stain) in the case of gonococcal urethritis involves preparing a smear of the discharge from the penis on a glass slide and staining

Positive cultures are confirmed by biochemical identification. Neisseria gonorrhoeae in pus from a case of gonorrhoea in a man (Gram stain) Gonorrhea is caused by the bacterium Neisseria gonorrhoeae. Previous infection does not confer immunity – a person who has been infected can become infected again by exposure to someone who is infected. Neisseria A genus of gram-positive, rod-shaped bacteria whose organisms are nonmotile. Filaments that may be present in certain species are either straight or wavy and may have swollen or clubbed heads. (Source: National Library of Medicine 2013 MeSH Scope Note and Classification) Neisseria gonorrhoeae is a Gram-negative coccus, 0.6 to 1.0 µm in diameter, usually seen in pairs with adjacent flattened sides (Figure 1 Left and Fig 2 below). The organism is frequently found intracellularly in polymorphonuclear leukocytes (neutrophils) of the gonorrhea pustular exudate (Figure 1 Right).

CULTURE Find neisseria gonorrhoeae stock images in HD and millions of other royalty-free stock photos, illustrations and vectors Gram stain for Neisseria gonorrhoeae. Gram-stain techniques in acute gonococcal u. gram-negative diplococci is increasing out of proportion to that caused by Neisseria gonorrhoeae. In the study You can use all images from this gallery for whatever you want, use it freely for personal and commercial use. photomicrograph, neisseria gonorrhoeae, gram, N. gonorrhoeae culture was performed when gram-negative diplococci were present in a gram-stained smear or with material collected at a return visit when the Urethral discharge in Gram stain. Urethral discharge in Gram stain is showing Gram negative diplococci intracellular as well as extracellular as shown above Gonorrhea is caused by the bacterium Neisseria gonorrhoeae. Previous Traditionally, gonorrhea was diagnosed with Gram stain and culture; however, newer Results: Greater than 100 WBC's/hpf || Numerous intracellular gram negative diplococci.

Figure 1. Left: Neisseria gonorrhoeae Gram stain of pure culture; Right: Neisseria gonorrhoeae Gram stain of a pustular exudate. Neisseria gonorrhoeae Neisseria gonorrhoeae is a Gram-negative coccus, 0.6 to 1.0 µm in diameter, usually seen in pairs with adjacent flattened sides (Figure 1 Left and Fig 2 below).

If urine NAAT is NOT available, recommend Gram Stain and urethral GC Note: All GC cultures presumptively identified as Neisseria gonorrhoeae must be.

In liquid culture media like peptone water and TS broth, the growth of the bacterium occurs as Granular turbidity in the broth medium which is further analyzed for the morphology (under the microscope), gram reaction, biochemical tests, and Neisseria gonorrhoeae specific tests. Neisseria gonorrhoeae gram staining of a preparation of urethral secretion showing coffee-bean-shaped diplococci, grouped within a granulocyte Neisseria gonorrhoeae Diagnosis Gram stain using methylene blue Neisseria gonorrhoeae bacteria revealed in the gram-stain image of a urethral discharge specimen, 1975.

Results: Greater than 100 WBC's/hpf || Numerous intracellular gram negative diplococci. Neisseria gonorrhoeae 01. Neisseria gonorrhoeae 02. Neisseria

Gram stain. Diphtheroids gram stain. Sputum gram stain interpretation.

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N. cinerea colonies are slightly more pigmented after incubation for 48 h. A direct smear for Gram staining may be performed as soon as the swab specimen is collected from the urethra, cervix, vagina or rectum. The Gram stain of a smear of urethral exudates or endocervical secretions shows typical Gram-negative, non-motile, intracellular diplococci. Gonorrhea cultures are incubated for a minimum of 48-72 hours. Growth on MTM is screened for presumptive positives by oxidase test and gram stain.

Figure 3. Gram stain of spun CSF showing N. meningitidis diplococci.
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In contrast to Neisseria spp., K. denitrificans isolates are catalase- and superoxol-negative. N. gonorrhoeae and K. denitrificans may, however, be confused because isolates of both species produce acid from glucose in acid production tests and hydroxylprolylaminopeptidase in enzyme substrate tests.

Gram negative diplococci (fastidious) Clinical Significance. This organism is associated with sexually transmitted infections including urethritis, epididymitis, orchitis, prostatitis, proctitis, cervicitis, salpingitis, pelvic inflammatory disease, Bartholin’s abscess, and pharyngitis. Gram stain Cell Morphology: of Neisseria spp. isolated on selective media for N. gonorrhoeae, isolates of other Neisseria spp.

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1 May 2013 A Gram stain of discharge shows gram-negative, intracellular diplococci. Neisseria gonorrhoeae was isolated on chocolate agar after

Limitations: Gram stains are not performed on anatomic loci that may contain non-gonococcal Neisseriae; such as respiratory, vaginal, cervical, and anal sources. The Gram stain is diagnostic only for male urethral specimens. Rates of recovery for N. gonorrhoeae can be significantly improved if specimen is received within 12 hours of collection. Gonorrhoea is a disease is due to infection with the bacteria Neisseria gonorrhoeae. The common sites of infection are the mucous membranes of the urethra, endocervix, rectum, pharynx and conjunctiva.